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Ogliari,Juliana Bernardi; Boscariol,Raquel L.; Camargo,Luis E.A.. |
Maize (Zea mays L.) microsatellite loci are useful as genetic markers because they are numerous, occur in every chromosome, and have a high content of polymorphism information. Furthermore, they can be amplified by PCR and the resulting fragments resolved on agarose gels. A major problem, however, is that the primers used in their amplification often have different length and nucleic acid content, requiring optimization of PCR amplification programs for each locus. We developed "touchdown" PCR programs successfully used in the amplification of a set of 125-maize microsatellite loci, chosen as representative of all chromosomes. The programs varied both in relation to the annealing temperature (Tm) and primer concentration. Primers could be divided into four... |
Tipo: Info:eu-repo/semantics/article |
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Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200026 |
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Bernardo Gongalves,Gábríel Moreno; Mayer,Luana Burg; Souza,Rosenilda de; Ogliari,Juliana Bernardi. |
Abstract The aim of this study was to evaluate grain yield (GY), popping expansion (PE), popping expansion disregarding the weight of unpopped kernels (PEW), and eleven other agronomic and morphological traits of 14 local popcorn varieties (LPV) from far west of the state of Santa Catarina, Southern Brazil. Two experiments were carried out, in a completely randomized block design with three replications, in different regions of Santa Catarina: one in the municipality of Florianópolis (east coast) and the other in Anchieta (western). Grain yield had the highest positive correlation with prolificacy (PRL) (r = 0.72) and number of grains per row (NGR) (r = 0.71), and a negative correlation with caryopsis circularity index (CCI) (r = -0.67). Variable PEW... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agrobiodiversity; Genetic potential; Landrace; Phenotypic correlation; Pre-breeding; Zea mays L. |
Ano: 2019 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122019000300213 |
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