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Chromosomal locations of the maize (Zea mays L.) HtP and rt genes that confer resistance to Exserohilum turcicum Genet. Mol. Biol.
Ogliari,Juliana Bernardi; Guimarães,Marco Antônio; Camargo,Luis Eduardo Aranha.
We used 125 microsatellite markers to genotype the maize (Zea mays L.) near isogenic lines (NIL) L30HtPHtPRtRt and L30htphtpRtRt and the L40htphtprtrt line which contrast regarding the presence of the recently described dominant HtP and the recessive rt genes that confer resistance to Exserohilum turcicum. Five microsatellite markers revealed polymorphisms between the NIL and were considered candidate linked markers for the HtP resistance gene. Linkage was confirmed by bulked segregant sample (BSS) analysis of 32 susceptible and 34 resistant plants from a BC1F1 population derived from the cross (L30HtPHtPRtRt x L40htphtprtrt) x L40htphtprtrt. The bnlg198 and dupssr25 markers, both located on maize chromosome 2L (bin 2.08), were polymorphic between bulks....
Tipo: Info:eu-repo/semantics/other Palavras-chave: Bulked segregant sample analysis; Microsatellite markers; Maize; Near-isogenic lines; Northern leaf blight.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400021
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Genetic variability for carotenoid content of grains in a composite maize population Scientia Agricola
Kist,Volmir; Albino,Vanessa Stainbach; Maraschin,Marcelo; Ogliari,Juliana Bernardi.
Local maize (Zea mays L.) varieties are cultivated by small-scale farmers in western Santa Catarina (SC) State, in southern Brazil. These small areas frequently present many problems related to biotic and non-biotic stresses, which have limited the economic output and income of the farmers. Production from local varieties for human consumption would be an alternative way of improving income and stimulating on farm conservation. The genetic variability of the total carotenoid content (TCC) of kernels in a local maize population was evaluated for their economic exploitation potential as biofortified food. Two independent samples of 96 half-sib families (HSF) plus four checks were evaluated in two groups of experiments in western SC and each one was carried...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofortified food; Convergent-divergent selection; Genetic-statistical parameters; Genotype by environment interaction.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000600006
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New resistance genes in the Zea mays: exserohilum turcicum pathosystem Genet. Mol. Biol.
Ogliari,Juliana Bernardi; Guimarães,Marco Antônio; Geraldi,Isaías Olívio; Camargo,Luis Eduardo Aranha.
The use of monogenic race-specific resistance is widespread for the control of maize (Zea mays L.) helminthosporiosis caused by Exserohilum turcicum. Inoculation of 18 Brazilian isolates of E. turcicum onto elite maize lines containing previously identified resistance genes and onto differential near-isogenic lines allowed the identification of new qualitative resistance genes. The inoculation of one selected isolate on differential near-isogenic lines, F1 generations and a BC1F1 population from the referred elite lines enabled the characterization of the resistance spectrum of three new genes, one dominant (HtP), one recessive (rt) and a third with non-identified genetic action. Three physiological races of the pathogen were also identified including two...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Northern leaf blight; Genetic inheritance; Resistance genes; Races; Maize.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300017
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Old tools as new support for on farm conservation of different types of maize Scientia Agricola
Vidal,Rafael; Silva,Natália Carolina de Almeida; Ogliari,Juliana Bernardi.
ABSTRACT: A germplasm collection should represent the diversity of the target species and the gene pools associated with it. However, it is critical to establish collection plans which ensure such representativeness. At times it is difficult to identify the best strategy for collecting domesticated species that are conserved in situ/on farm, since, in general, the magnitude of the diversity existing in a geographical area was hitherto unknown. The Diversity Census methodology was developed for previous diagnosis of the diversity of Zea mays subsp. mays L., conserved by farmers in two municipalities in the far western region of the state of Santa Catarina, southern Brazil. The Diversity Census database allowed for the identification of the best strategy to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays L.; Genetic diversity; Morphological characters; Use values.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000101100
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Optimization of PCR amplification of maize microsatellite loci Genet. Mol. Biol.
Ogliari,Juliana Bernardi; Boscariol,Raquel L.; Camargo,Luis E.A..
Maize (Zea mays L.) microsatellite loci are useful as genetic markers because they are numerous, occur in every chromosome, and have a high content of polymorphism information. Furthermore, they can be amplified by PCR and the resulting fragments resolved on agarose gels. A major problem, however, is that the primers used in their amplification often have different length and nucleic acid content, requiring optimization of PCR amplification programs for each locus. We developed "touchdown" PCR programs successfully used in the amplification of a set of 125-maize microsatellite loci, chosen as representative of all chromosomes. The programs varied both in relation to the annealing temperature (Tm) and primer concentration. Primers could be divided into four...
Tipo: Info:eu-repo/semantics/article
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200026
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Yield and popping expansión components in local popcorn varieties from Southern Brazil Acta Agron. (Palmira)
Bernardo Gongalves,Gábríel Moreno; Mayer,Luana Burg; Souza,Rosenilda de; Ogliari,Juliana Bernardi.
Abstract The aim of this study was to evaluate grain yield (GY), popping expansion (PE), popping expansion disregarding the weight of unpopped kernels (PEW), and eleven other agronomic and morphological traits of 14 local popcorn varieties (LPV) from far west of the state of Santa Catarina, Southern Brazil. Two experiments were carried out, in a completely randomized block design with three replications, in different regions of Santa Catarina: one in the municipality of Florianópolis (east coast) and the other in Anchieta (western). Grain yield had the highest positive correlation with prolificacy (PRL) (r = 0.72) and number of grains per row (NGR) (r = 0.71), and a negative correlation with caryopsis circularity index (CCI) (r = -0.67). Variable PEW...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agrobiodiversity; Genetic potential; Landrace; Phenotypic correlation; Pre-breeding; Zea mays L.
Ano: 2019 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122019000300213
Registros recuperados: 6
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